genbank database Search Results


the streptomyces spomt2884 gene (genbank protein database accession number kf420279)  (GenScript corporation)
90
GenScript corporation the streptomyces spomt2884 gene (genbank protein database accession number kf420279)
Construction and expression of <t>SpOMT2884</t> in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.
The Streptomyces Spomt2884 Gene (Genbank Protein Database Accession Number Kf420279), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gfap (gi:164694994 in the genbank database)  (US Biological Life Sciences)
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US Biological Life Sciences gfap (gi:164694994 in the genbank database)
Construction and expression of <t>SpOMT2884</t> in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.
Gfap (Gi:164694994 In The Genbank Database), supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleotide databases genbank  (Incyte corporation)
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Incyte corporation nucleotide databases genbank
Construction and expression of <t>SpOMT2884</t> in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.
Nucleotide Databases Genbank, supplied by Incyte corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleotide databases genbank - by Bioz Stars, 2026-08
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ncbi genbank database  (PrimerDesign Inc)
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PrimerDesign Inc ncbi genbank database
Construction and expression of <t>SpOMT2884</t> in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.
Ncbi Genbank Database, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ncbi genbank database - by Bioz Stars, 2026-08
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genbank wholegenome shotgun (wgs) database  (WholeGenome LLC)
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WholeGenome LLC genbank wholegenome shotgun (wgs) database
Construction and expression of <t>SpOMT2884</t> in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.
Genbank Wholegenome Shotgun (Wgs) Database, supplied by WholeGenome LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chinese hamster ovary cells (cho-k1)  (Verlag GmbH)
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Verlag GmbH chinese hamster ovary cells (cho-k1)
Construction and expression of <t>SpOMT2884</t> in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.
Chinese Hamster Ovary Cells (Cho K1), supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chinese hamster ovary cells (cho-k1) - by Bioz Stars, 2026-08
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materials gfap (gi:164694994 in the genbank database) from normal human brain  (US Biological Life Sciences)
90
US Biological Life Sciences materials gfap (gi:164694994 in the genbank database) from normal human brain
Construction and expression of <t>SpOMT2884</t> in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.
Materials Gfap (Gi:164694994 In The Genbank Database) From Normal Human Brain, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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materials gfap (gi:164694994 in the genbank database) from normal human brain - by Bioz Stars, 2026-08
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genbank database  (Clinical and Laboratory Standards Institute)
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Clinical and Laboratory Standards Institute genbank database
Construction and expression of <t>SpOMT2884</t> in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.
Genbank Database, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genbank database - by Bioz Stars, 2026-08
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genbank database  (Future Medicine Ltd)
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Future Medicine Ltd genbank database
Construction and expression of <t>SpOMT2884</t> in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.
Genbank Database, supplied by Future Medicine Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genbank database - by Bioz Stars, 2026-08
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genbank nucleotide sequence database  (Sherman Fairchild Foundation)
90
Sherman Fairchild Foundation genbank nucleotide sequence database
Construction and expression of <t>SpOMT2884</t> in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.
Genbank Nucleotide Sequence Database, supplied by Sherman Fairchild Foundation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genbank nucleotide sequence database - by Bioz Stars, 2026-08
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genbank database  (Informa UK Limited)
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Informa UK Limited genbank database
Construction and expression of <t>SpOMT2884</t> in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.
Genbank Database, supplied by Informa UK Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mbl/genbank database of virus sequences  (MBL Life science)
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MBL Life science mbl/genbank database of virus sequences
Construction and expression of <t>SpOMT2884</t> in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.
Mbl/Genbank Database Of Virus Sequences, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Construction and expression of SpOMT2884 in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.

Journal: International Journal of Molecular Sciences

Article Title: Production of Two Novel Methoxy-Isoflavones from Biotransformation of 8-Hydroxydaidzein by Recombinant Escherichia coli Expressing O -Methyltransferase SpOMT2884 from Streptomyces peucetius

doi: 10.3390/ijms161126070

Figure Lengend Snippet: Construction and expression of SpOMT2884 in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.

Article Snippet: The Streptomyces SpOMT2884 gene (GenBank protein database accession number KF420279) was chemically synthesized with codon optimization based on the preferences of E. coli by GenScript (Piscataway, NJ, USA).

Techniques: Expressing, Plasmid Preparation, Polyacrylamide Gel Electrophoresis, SDS Page, Recombinant, Lysis, Molecular Weight

Ultra-performance liquid chromatography (UPLC) profiles of 8-hydroxydaidzein standard ( A ) and fermentation broth (( B ): 0 h induction; ( C ): 24 h induction) of recombinant E. coli expressing SpOMT2884. The recombinant strain was cultivated at 37 °C in shacking flasks with LeMaster and Richards minimal medium (LR medium) containing 100 μM 8-hydroxydaidzein. After induction with 0.5 mM isopropyl-β- d -thiogalactopyranoside (IPTG), aliquot samples of the fermentation were collected and analyzed with UPLC. The detailed protocols for fermentation and UPLC are described in Materials and Methods.

Journal: International Journal of Molecular Sciences

Article Title: Production of Two Novel Methoxy-Isoflavones from Biotransformation of 8-Hydroxydaidzein by Recombinant Escherichia coli Expressing O -Methyltransferase SpOMT2884 from Streptomyces peucetius

doi: 10.3390/ijms161126070

Figure Lengend Snippet: Ultra-performance liquid chromatography (UPLC) profiles of 8-hydroxydaidzein standard ( A ) and fermentation broth (( B ): 0 h induction; ( C ): 24 h induction) of recombinant E. coli expressing SpOMT2884. The recombinant strain was cultivated at 37 °C in shacking flasks with LeMaster and Richards minimal medium (LR medium) containing 100 μM 8-hydroxydaidzein. After induction with 0.5 mM isopropyl-β- d -thiogalactopyranoside (IPTG), aliquot samples of the fermentation were collected and analyzed with UPLC. The detailed protocols for fermentation and UPLC are described in Materials and Methods.

Article Snippet: The Streptomyces SpOMT2884 gene (GenBank protein database accession number KF420279) was chemically synthesized with codon optimization based on the preferences of E. coli by GenScript (Piscataway, NJ, USA).

Techniques: Liquid Chromatography, Recombinant, Expressing

Diagram of the biotransformation of 8-hydroxydaidzein by the recombinant E. coli expressing SpOMT2884.

Journal: International Journal of Molecular Sciences

Article Title: Production of Two Novel Methoxy-Isoflavones from Biotransformation of 8-Hydroxydaidzein by Recombinant Escherichia coli Expressing O -Methyltransferase SpOMT2884 from Streptomyces peucetius

doi: 10.3390/ijms161126070

Figure Lengend Snippet: Diagram of the biotransformation of 8-hydroxydaidzein by the recombinant E. coli expressing SpOMT2884.

Article Snippet: The Streptomyces SpOMT2884 gene (GenBank protein database accession number KF420279) was chemically synthesized with codon optimization based on the preferences of E. coli by GenScript (Piscataway, NJ, USA).

Techniques: Recombinant, Expressing